Textbook in PDF format
This volume explores the latest techniques used to study how mesoscale imaging can be applied in various areas of neuroscientific research. The chapters in this book are organized into four parts: Part One highlights novel imaging modalities, such as neurotransmitter and single-cell resolution imaging and analyses of neuroimaging data in awake rodents. Part Two covers technical innovations such as head-mounted camera systems, multi-site photometry, and concurrent imaging-electrographic probes. Part Three presents methods of mesoscale imaging during normal behavior, and Part Four looks at the mesoscale imaging methods used to study neurological diseases and disorders that are critical to observe in the non-anesthetized condition. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Awake Behaving Mesoscopic Brain Imaging is a valuable resource for both novice and expert researchers who are interested in learning more about this exciting and innovative topic.
Preface to the Series
Preface
Contributors
Imaging Modalities and Analyses
Mesoscopic Imaging of Neurotransmitters and Neuromodulators with Genetically Encoded Sensors
Analysis of Mesoscope Imaging Data
Real-Time Ultra-Large-Scale Imaging with High-Resolution Microscopy
Mesoscale Imaging Techniques
Mesoscale Calcium (Ca2+) Imaging in Freely Behaving Mice
High-Density Multichannel Fiber Photometry
Widefield Imaging Combined with a Transparent Electrographic Probe
Imaging Behavior and Motor Systems
Wide-Field Calcium Imaging of Mesoscale Networks Underlying the Encoding of Skilled Voluntary Movement
Technical Considerations for Widefield Optical Imaging During Visuomotor Behaviors
Mesoscale Ca2+ Imaging During Locomotion
Mesoscale Imaging of Brain Disease
Mesoscale Ca++ Imaging of Seizures and Interictal Activity in Models of Chronic Epilepsy
Mesoscale Imaging of Neural Dynamics in Epilepsy
Mesoscale Imaging of Stroke
Index