Loughran S. Protein Chromatography. Methods and Protocols 3ed 2023 torrent

Textbook in PDF format

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.
A Synopsis of Proteins and Their Purification
Mixed-Mode Chromatography and Its Role in Monoclonal Antibody Purification
Continuous Countercurrent Chromatography in Protein Purification
Chromatographic Purification of Viral Vectors for Gene Therapy Applications
Scale-Up of Protein Purification:​ Downstream Processing Issues
Approaches to Avoid Proteolysis During Protein Expression and Purification
Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Magnetic Nanoparticles for Protein Separation and Purification
Ion-Exchange Chromatography:​ Basic Principles and Application
Hydroxyapatite Chromatography (HAC)
Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC)
Lectin Affinity Chromatography
Single-Step Non-Chromatographic Purification of Recombinant Mammalian Proteins Using a Split Intein ELP Tag System
Clinical Proteomics:​ Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems
Immunoprecipitat​ion:​ Variations, Considerations, and Applications
Protein Quantitation and Analysis of Purity
Protein Extraction and Purification by Differential Solubilization
Protein Stability:​ Enhancement and Measurement
Storage and Lyophilization of Pure Proteins
Strategies for the Purification of Membrane Proteins